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PerSeptive Biosystems Inc automated nucleic acid synthesizer expedite 8909
Automated Nucleic Acid Synthesizer Expedite 8909, supplied by PerSeptive Biosystems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expedite+8909+synthesizer/expedite+8909+automated+dna+synthesizer/pmc10591465__ja3c06500_si_002-37-12-16
Average 90 stars, based on 1 article reviews
automated nucleic acid synthesizer expedite 8909 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Concentration Assay:

Article Title: Engineering disulfide cross-links in RNA via air oxidation.
Article Snippet: .. This amidite concentration is recommended for an Expedite 8909 synthesizer (Perseptive Biosystems). ..

Article Title: Endonuclease-substrate complexes
Article Snippet: .. Invader assay reaction mixtures were set up as follows: Reaction component Stock concentration Final concentration Pfu FEN 1 10 nM 1 nM R94A variant of Pfu 44.7 μM 4.47 μM FEN1 Invader 2 μM 500 nM oligo/probe/target mix 10 X DNA reaction 10X 1X buffer 1 Final volume 100 μl Oligonucleotides were synthesized on an Expedite 8909 synthesizer (PerSeptive Biosystems) using standard phosphoramidite chemistry. ..

Incubation:

Article Title: Sequence and structure analysis of a mirror tRNA located upstream of the cytochrome oxidase I mRNA in mouse mitochondria.
Article Snippet: RNA fragments corresponding to the mirror tRNA that is located upstream of the cytochrome oxidase I (COXI) gene in the mouse mitochondrial genome were found in the sequences obtained from the mouse brain by the next generation sequencing.. RNA fragments corresponding to the 50 terminal of COXI mRNA were also found and it was suggested that the precursor of the COXI mRNA is processed at three residues upstream of the first AUG codon.. The mirror tRNA fragment has poly(A) in its 30 terminal and variable 50 terminal, suggesting that this RNA is produced during the 50 processing of COXI mRNA.

Synthesized:

Article Title: Sequence and structure analysis of a mirror tRNA located upstream of the cytochrome oxidase I mRNA in mouse mitochondria.
Article Snippet: RNA fragments corresponding to the mirror tRNA that is located upstream of the cytochrome oxidase I (COXI) gene in the mouse mitochondrial genome were found in the sequences obtained from the mouse brain by the next generation sequencing.. RNA fragments corresponding to the 50 terminal of COXI mRNA were also found and it was suggested that the precursor of the COXI mRNA is processed at three residues upstream of the first AUG codon.. The mirror tRNA fragment has poly(A) in its 30 terminal and variable 50 terminal, suggesting that this RNA is produced during the 50 processing of COXI mRNA.

Article Title: Endonuclease-substrate complexes
Article Snippet: .. Invader assay reaction mixtures were set up as follows: Reaction component Stock concentration Final concentration Pfu FEN 1 10 nM 1 nM R94A variant of Pfu 44.7 μM 4.47 μM FEN1 Invader 2 μM 500 nM oligo/probe/target mix 10 X DNA reaction 10X 1X buffer 1 Final volume 100 μl Oligonucleotides were synthesized on an Expedite 8909 synthesizer (PerSeptive Biosystems) using standard phosphoramidite chemistry. ..

Article Title: Endonuclease-substrate complexes
Article Snippet: .. Materials and Methods DNA synthesis and purification: DNA oligonucleotides were synthesized with an Expedite 8909 synthesizer (PerSeptive Biosystems) using standard phosphoramidite chemistry. .. All phosphoramidites including tetracholorofluorecsein (TET) dye, 2′-O-methyl, and methylphosphonate phosphoramidites were purchased from Glen Research, Sterling, Va. Synthesis and deprotection were performed according to the vendor's recommended procedures and further purification was performed by electrophoresis on a 20% denaturing acrylamide gel as described (Kaiser et al., 1999).

Article Title: Differences in uptake, localization, and processing of PNAs modified by COX VIII pre-sequence peptide and by triphenylphoshonium cation into mitochondria of tumor cells.
Article Snippet: Two approaches to target PNAs (peptide nucleic acids) into mitochondria of HeLa cells were compared.. In the first, PNA was modified with the lipophilic cation TPP.. TPP-PNA accumulated rapidly within mitochondria driven by the membrane potential.

Article Title: Reversible photoswitching of RNA hybridization at room temperature with an azobenzene C-nucleoside.
Article Snippet: Photoregulation of RNA remains a challenging task as the introduction of a photoswitch entails changes in the shape and the stability of the duplex that strongly depend on the chosen linker strategy.. Herein, the influence of a novel nucleosidic linker moiety on the photoregulation efficiency of azobenzene is investigated.. To this purpose, two azobenzene C-nucleosides were stereoselectively synthesized, characterized, and incorporated into RNA oligonucleotides.

Article Title: Super-resolution labelling with Action-PAINT
Article Snippet: .. Synthesis of CNVK-bearing and fluorophore-labelled oligonucleotide Oligonucleotides carrying a 3-Cyanovinylcarbazole (CNVK) modified base were synthesized using standard phosphoramidite chemistry adapted from previous report4 on an Expedite 8909 synthesizer (Perseptive Biosystems). ..

Variant Assay:

Article Title: Endonuclease-substrate complexes
Article Snippet: .. Invader assay reaction mixtures were set up as follows: Reaction component Stock concentration Final concentration Pfu FEN 1 10 nM 1 nM R94A variant of Pfu 44.7 μM 4.47 μM FEN1 Invader 2 μM 500 nM oligo/probe/target mix 10 X DNA reaction 10X 1X buffer 1 Final volume 100 μl Oligonucleotides were synthesized on an Expedite 8909 synthesizer (PerSeptive Biosystems) using standard phosphoramidite chemistry. ..

DNA Synthesis:

Article Title: Endonuclease-substrate complexes
Article Snippet: .. Materials and Methods DNA synthesis and purification: DNA oligonucleotides were synthesized with an Expedite 8909 synthesizer (PerSeptive Biosystems) using standard phosphoramidite chemistry. .. All phosphoramidites including tetracholorofluorecsein (TET) dye, 2′-O-methyl, and methylphosphonate phosphoramidites were purchased from Glen Research, Sterling, Va. Synthesis and deprotection were performed according to the vendor's recommended procedures and further purification was performed by electrophoresis on a 20% denaturing acrylamide gel as described (Kaiser et al., 1999).

Purification:

Article Title: Endonuclease-substrate complexes
Article Snippet: .. Materials and Methods DNA synthesis and purification: DNA oligonucleotides were synthesized with an Expedite 8909 synthesizer (PerSeptive Biosystems) using standard phosphoramidite chemistry. .. All phosphoramidites including tetracholorofluorecsein (TET) dye, 2′-O-methyl, and methylphosphonate phosphoramidites were purchased from Glen Research, Sterling, Va. Synthesis and deprotection were performed according to the vendor's recommended procedures and further purification was performed by electrophoresis on a 20% denaturing acrylamide gel as described (Kaiser et al., 1999).

Modification:

Article Title: Super-resolution labelling with Action-PAINT
Article Snippet: .. Synthesis of CNVK-bearing and fluorophore-labelled oligonucleotide Oligonucleotides carrying a 3-Cyanovinylcarbazole (CNVK) modified base were synthesized using standard phosphoramidite chemistry adapted from previous report4 on an Expedite 8909 synthesizer (Perseptive Biosystems). ..



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